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Characterization and genome sequencing of a novel bacteriophage infecting Streptococcus agalactiae with high similarity to a phage from Streptococcus pyogenes.
- Source :
- Archives of Virology; Aug2013, Vol. 158 Issue 8, p1733-1741, 9p, 1 Black and White Photograph, 2 Diagrams, 3 Graphs
- Publication Year :
- 2013
-
Abstract
- A novel bacteriophage, JX01, specifically infecting bovine Streptococcus agalactiae was isolated from milk of mastitis-affected cattle. The phage morphology showed that JX01 belongs to the family Siphoviridae, and this phage demonstrated a broad host range. Microbiological characterization demonstrated that nearly 90 % of JX01 phage particles were adsorbed after 2.5 min of incubation, that the burst size was 20 virions released per infected host cell, and that there was a latent period of 30 min. JX01 was thermal sensitive and showed acid and alkaline resistance (pH 3-11). The genome of JX01 was found to consist of a linear, double-stranded 43,028-bp DNA molecule with a GC content of 36.81 % and 70 putative open reading frames (ORFs) plus one tRNA. Comparative genome analysis revealed high similarity between JX01 and the prophage 315.2 of Streptococcus pyogenes. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03048608
- Volume :
- 158
- Issue :
- 8
- Database :
- Complementary Index
- Journal :
- Archives of Virology
- Publication Type :
- Academic Journal
- Accession number :
- 89397275
- Full Text :
- https://doi.org/10.1007/s00705-013-1667-x