Preliminary study on the discrimination of oral mucosa pathogens Saccharomyces with metabonomics method.

Objective To evaluate the feasibility of identifying oral mucosa pathogenic bacteria by comparing the metabolic profiling of Sciccharomyces and try to find a convenient and rapid way to discriminate oral micro-organisms. Methods Saccharomyces albicams ATCC 10231 and Saccharomyces tropicalis ATCC 13803 and Saccharomyces glabrata ATCC 15126 were respectively inoculated in sabouraud medium. The growth quantity were measured periodically by a spectrophotometer. And the growth curves of the inoculated bacteria were completed. The culture solutions in stationary phases of the three bacteria were respectively tested with ¹H-nuclear magnetic resonance (¹H-NMR) spectroscopy. The data of H-NMR spectroscopy results were analyzed by principal components analysis (PCA). Results The PCA showed respectively a obvious clustering phenomena and the points of every two groups' data stayed separately. Therefore, the NMR-based metabonornics profiles could discriminate the three kinds of mucosa pathogenic bacteria. Conclusion The metabonomics can be expected to be a kind of promising useful method in quick discrimination of oral pathogenic bacteria. [ABSTRACT FROM AUTHOR]