CD4+ T regulatory cells from the colonic lamina propria of normal mice inhibit proliferation of enterobacteria-reactive, disease-inducing Th1-cells from scid mice with colitis.

SUMMARY Adoptive transfer of CD4⁺ T cells into scid mice leads to a chronic colitis in the recipients. The transferred CD4⁺ T cells accumulate in the intestinal lamina propria (LP), express an activated Th1 phenotype and proliferate vigously when exposed ex vivo to enteric bacterial antigens. As LP CD4⁺ T cells from normal BALB/c mice do not respond to enteric bacterial antigens, we have investigated whether colonic LP-derived CD4⁺ T cells from normal mice suppress the antibacterial response of CD4⁺ T cells from scid mice with colitis. LP-derived CD4⁺ T cells cocultured with bone marrow-derived dendritic cells effectively suppress the antibacterial proliferative response of CD4⁺ T cells from scid mice with colitis. The majority of these LP T-reg cells display a nonactivated phenotype and suppression is independent of antigen exposure, is partly mediated by soluble factor(s) different from IL-10 and TGF-β , and is not prevented by the addition of high doses of IL-2 to the assay culture. Functionally and phenotypically the T-reg cells of the present study differ from previously described subsets of T-reg cells. The presence of T cells with a regulatory potential in the normal colonic mucosa suggests a role for these cells in the maintenance of local immune homeostasis of the gut. [ABSTRACT FROM AUTHOR]