Back to Search
Start Over
Determination of estradiol valerate in pharmaceutical preparations and human serum by flow injection chemiluminescence.
- Source :
- Luminescence: Journal of Biological & Chemical Luminescence; May2013, Vol. 28 Issue 3, p407-411, 5p
- Publication Year :
- 2013
-
Abstract
- ABSTRACT A novel method for the detection of trace estradiol valerate (EV) in pharmaceutical preparations and human serum was developed by inhibition of luminol chemiluminescence (CL) by estradiol valerate on the zinc deuteroporphyrin (ZnDP)-enhanced luminol-K<subscript>3</subscript>Fe(CN)<subscript>6</subscript> chemiluminescence system. Under optimized experimental conditions, CL intensity and concentration of estradiol valerate had a good linear relationship in the ranges of 8.0 × 10<superscript>-8</superscript> to 1.0 × 10<superscript>-5</superscript> g/mL. Detection limit (3 σ) was estimated to be 3.5 × 10<superscript>-8</superscript> g/mL. The proposed method was applied successfully for the determination of estradiol valerate in pharmaceutical preparations and human serum and recoveries were 97.0-105.0% and 95.5-106.0%, respectively. The possible mechanism of the CL system is discussed. Copyright © 2012 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15227235
- Volume :
- 28
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Luminescence: Journal of Biological & Chemical Luminescence
- Publication Type :
- Academic Journal
- Accession number :
- 88059247
- Full Text :
- https://doi.org/10.1002/bio.2397