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Expression of blaA Underlies Unexpected Ampicillin-Induced Cell Lysis of Shewanella oneidensis.

Expression of blaA Underlies Unexpected Ampicillin-Induced Cell Lysis of Shewanella oneidensis.

Authors :
Yin, Jianhua
Sun, Linlin
Dong, Yangyang
Chi, Xun
Zhu, Weiming
Qi, Shu-hua
Gao, Haichun
Source :
PLoS ONE; Mar2013, Vol. 8 Issue 3, p1-10, 10p
Publication Year :
2013

Abstract

Shewanella oneidensis is a facultative anaerobic γ-proteobacterium possessing remarkably diverse respiratory capacities for reducing various organic and inorganic substrates. As a veteran research model for investigating redox transformations of environmental contaminants the bacterium is well known to be a naturally ampicillin-resistant microorganism. However, in this study we discovered that ampicillin has a significant impact on growth of S. oneidensis. Particularly, cell lysis occurred only with ampicillin at levels ranging from 0.49 to 6.25 µg/ml but not at 50 µg/ml. This phenotype is attributable to insufficient expression of the β-lactamase BlaA. The subsequent analysis revealed that the blaA gene is strongly induced by ampicillin at high (50 µg/ml), but not at low levels (2.5 µg/ml). In addition, we demonstrated that penicillin binding protein 5 (PBP5), the most abundant low molecular weight PBP (LMW PBP), is the only one relevant to β-lactam resistance under the tested conditions. This nonessential PBP, largely resembling its Escherichia coli counterpart in functionality, mediates expression of the blaA gene. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
19326203
Volume :
8
Issue :
3
Database :
Complementary Index
Journal :
PLoS ONE
Publication Type :
Academic Journal
Accession number :
87681967
Full Text :
https://doi.org/10.1371/journal.pone.0060460