Study on oxidative damage of mouse lung cells induced by pesticide chlorpyrifos.

Chlorpyrifos is one kind of efficient, broad-spectrum organic phosphorus insecticide. This study aims at observing the oxidative stress effects of chlorpyrifos on organisms. Kunming mice were orally administrated with chlorpyrifos daily for seven days at the concentration of 3, 6 and 12 mg/kg respectively, Afterwards, organ coefficient ratio of mouse lung were measured, the contents of ROS (reactive oxygen species),GSH (glutathione) and MDA(Malondialdehyde) in the lung tissue homogenates were measured. The experimental results showed that the contents of ROS and MDA coefficients increased gradually while Organ coefficient ratio and GSH content decreased with the increasing exposure dose, all the biomarkers were in the exposure dose-response manner. When the exposure dose was over 6 mg/kg, GSH contents and MDA contents were significantly higher than the control group (P<C0. 05) ; in the higher dose groups (≥12 mg/kg), ROS contents was significantly higher than that of the control group (P<C0. 05), Organ coefficient ratio of mouse lung,GSH and MDA contents had extremely significant differences (P<C0. 01). These experimental results demonstrate that chlorpyrifos can increase the oxidative stress in mouse lung at higher dose. [ABSTRACT FROM AUTHOR]