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miR-21 represses Pdcd4 during cardiac valvulogenesis.
- Source :
- Development (09501991); 5/15/2013, Vol. 140 Issue 10, p2172-2180, 9p
- Publication Year :
- 2013
-
Abstract
- The discovery of small non-coding microRNAs has revealed novel mechanisms of post-translational regulation of gene expression, the implications of which are still incompletely understood. We focused on microRNA 21 (miR-21), which is expressed in cardiac valve endothelium during development, in order to better understand its mechanistic role in cardiac valve development. Using a combination of in vivo gene knockdown in zebrafish and in vitro assays in human cells, we show that miR-21 is necessary for proper development of the atrioventricular valve (AV). We identify pdcd4b as a relevant in vivo target of miR-21 and show that protection of pdcd4b from miR-21 binding results in failure of AV development. In vitro experiments using human pulmonic valve endothelial cells demonstrate that miR-21 overexpression augments endothelial cell migration. PDCD4 knockdown alone was sufficient to enhance endothelial cell migration. These results demonstrate that miR-21 plays a necessary role in cardiac valvulogenesis, in large part due to an obligatory downregulation of PDCD4. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09501991
- Volume :
- 140
- Issue :
- 10
- Database :
- Complementary Index
- Journal :
- Development (09501991)
- Publication Type :
- Academic Journal
- Accession number :
- 87467464
- Full Text :
- https://doi.org/10.1242/dev.084475