CHIRAL SEPARATION OF SEVERAL THIOBARBITURATES ON A CELLULOSE TRIS(4-METHYLBENZOATE) PHASE UNDER REVERSED-PHASE CONDITIONS.

The chiral separation of several thiobarbiturates on a tris(4-methylbenzoate) phase (Chiralcel OJ-R, 15 × 0.46 cm and Chiralcel OJ-R 15 × 0.20 cm) under reversed-phase conditions is described. The separation properties of a normal sized and a newly developed narrow-bore column were compared. The influence of buffer and organic modifiers on enantioselectvity was investigated. The three mobile phases used were water/acetonitrile (70:30,v/v), water/acetonitrile/methanol (50:25:25, v/v), and water/acetonitrile/methanol (55:15:30, v/v). Of seven thiobarbiturates investigated, five have been resolved. The values of α (1.11–1.85) and Rs (0.71–5.75) have been reported. [ABSTRACT FROM AUTHOR]