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Inhibition of Monocarboxylate Transporter-Mediated Absorption of Valproic Acid by Gegen-Qinlian-Tang.
- Source :
- American Journal of Chinese Medicine; 2013, Vol. 41 Issue 2, p369-378, 10p, 1 Diagram, 1 Chart, 2 Graphs
- Publication Year :
- 2013
-
Abstract
- Valproic acid (VPA), an anti-epileptic drug with a narrow therapeutic index, is a substrate of the monocarboxylate transporter (MCT). In this study, we investigated the effect of Gegen-Qinlian-Tang (GQT), a Chinese Medicine prescription containing Puerariae Radix (PR), Scutellariae Radix (SR), Coptidis Rhizoma (CR) and Glycyrrhizae Radix (GR), on the pharmacokinetics of VPA, as a probe drug of MCT, in rats and the underlying mechanism. Sprague-Dawley rats were orally administered VPA with and without GQT in crossover design. The serum concentrations of VPA were determined by a fluorescence polarization immunoassay. The results showed that coadministration with 2.0 and 4.0 g/kg of GQT remarkably decreased the C<subscript>max</subscript> of VPA by 72% and 74% and reduced the <subscript>0-t</subscript> by 63% and 53%, respectively. The mechanism study using Caco-2 cells revealed that the uptake function of MCT was inhibited by GQT and each component herb. In conclusion, the MCT-mediated absorption of VPA was significantly decreased by GQT and its component herbs. [ABSTRACT FROM AUTHOR]
- Subjects :
- CHINESE medicine
ANALYSIS of variance
ANIMAL experimentation
ANISOTROPY
CELL lines
CROSSOVER trials
FLUORIMETRY
HIGH performance liquid chromatography
DRUG-herb interactions
MEDICINAL plants
MOLECULAR structure
RATS
RESEARCH funding
STATISTICS
T-test (Statistics)
VALPROIC acid
DATA analysis
DATA analysis software
DESCRIPTIVE statistics
FLUORESCENT dyes
Subjects
Details
- Language :
- English
- ISSN :
- 0192415X
- Volume :
- 41
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- American Journal of Chinese Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 86648816
- Full Text :
- https://doi.org/10.1142/S0192415X13500274