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Two new compounds with cytotoxic activity on the human melanoma A375-S2 cells from Daphne giraldii callus cells.
- Source :
- Journal of Asian Natural Products Research; Nov2012, Vol. 14 Issue 11, p1020-1026, 7p, 3 Diagrams, 1 Chart
- Publication Year :
- 2012
-
Abstract
- Four compounds were isolated from Daphne giraldii callus cells, and their structures were characterized as daphnenone (1), daphnolon (2), R-(−)-1-(4′-hydroxyphenyl)-3-hydroxy-5-phenyl-1,5-pentandione (3), and S-(+)-daphneolone-4′-O-β-d-glucoside (4) on the basis of MS, NMR spectrographic analysis, and chemical methods. All of the four compounds possessed C6-C5-C6 carbon skeleton, and among them, 3 and 4 were two new compounds. In activity screening test, compounds 1, 2, 3, and 4 showed different degrees of cytotoxic activity against the tumor cells of human melanoma A375-S2 by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium hydrobromide method, with IC<subscript>50</subscript> values of 29.8, 51.0, 41.0, and 150.0 μM, respectively. Furthermore, we found the important target which could explain the cytotoxic mechanism of the four compounds by using autodock 4.0, a structure-guided discovery approach, and the important residues CYS532, GLY534, and SER535 of B-Raf kinase have been discovered. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10286020
- Volume :
- 14
- Issue :
- 11
- Database :
- Complementary Index
- Journal :
- Journal of Asian Natural Products Research
- Publication Type :
- Academic Journal
- Accession number :
- 84590321
- Full Text :
- https://doi.org/10.1080/10286020.2012.701206