Involvement of the alternative oxidase in cellular energy productionin the wheat 'take-all' fungus, Gaeumannomyces graminis var tritici (Extended summary)

Trichothecene resistance is achieved via 3-O-acetylation on the biosynthetic pathway of deoxynivalenol in Fusarium graminearum. The responsible 3-O-acetyltransferase gene, Tri101, was located between the UTP-ammonia ligase gene and the phosphate permease gene, and not in thetrichothecene biosynthetic gene cluster. As predicted by the presence of its homologues in yeasts, the resistance gene proved to have evolved independently of other biosynthetic genes. In Fusarium sporotrichioides, FsTri101 (a functional homologue of Tri101) was also locatedthese two 'house-keeping' genes. However, FsTri101 appeared not to play a pivotal role for self-resistance, suggesting that other defensive options are the primary working strategies for the type A trichothecene producer. (c) 1999 Society of Chemical Industry [ABSTRACT FROM AUTHOR]