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Molecular cloning and characterization of a microsatellite locus found in an RAPD marker of a spider mite, Panonychus citri (acari: tetranychidae)
- Source :
- Experimental & Applied Acarology; May1999, Vol. 23 Issue 5, p385, 0p
- Publication Year :
- 1999
-
Abstract
- Genetic markers were searched using PCR with 40 kinds of decanucleotide primers to investigate DNA polymorphism in Panonychuscitri. A region consisting of a variable number of CT tandem repeats (microsatellite) was found in a fragment amplified with the OPB10 primer. The microsatellite differed in size by ca. 100 bp among several P. citri populations screened and was derived from at least seven alleles. This region was characteristic of P. mori and P. osmanthi, but was lacking in P. ulmi. The flanking regions were highly conserved among these species. [ABSTRACT FROM AUTHOR]
- Subjects :
- MOLECULAR cloning
POLYMERASE chain reaction
MITES
GENETICS
DNA
ACAROLOGY
SPIDER mites
Subjects
Details
- Language :
- English
- ISSN :
- 01688162
- Volume :
- 23
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Experimental & Applied Acarology
- Publication Type :
- Academic Journal
- Accession number :
- 8384340