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Intra-individual variability of serum hepcidin-25 in haemodialysis patients using mass spectrometry and ELISA.
- Source :
- Nephrology Dialysis Transplantation; Oct2012, Vol. 27 Issue 10, p3923-3929, 7p, 3 Charts, 6 Graphs
- Publication Year :
- 2012
-
Abstract
- Background Measurement of serum hepcidin levels may provide a useful alternative to the current methods of determining iron status in chronic haemodialysis (HD) patients. However, the biological variability of this pivotal regulator of iron homeostasis is unclear, and the impact of inflammation, dialysis clearance and iron therapy on hepcidin variability has not been established. Methods Two independent studies in chronic HD patients were conducted; serum hepcidin levels were measured at the start of dialysis sessions in 20 UK patients and in 43 Dutch patients by mass spectrometry (MS). Samples from UK patients were also analysed by a competitive enzyme-linked immunosorbent assay (cELISA). Coefficient of variance (CV1) was calculated and potential factors affecting CV1 were also examined. Results The median CV1 (inter-quartile range) was 23% (17–28) for the UK MS, 26% (17–48) for the Dutch MS and 23% (17–39) for the UK cELISA. The CV1 was similar in those patients receiving and those not receiving regular intravenous iron. The CV1 was not associated with the degree of inflammation. Hepcidin levels were higher following an inter-dialytic period of 3 versus 2 days (P = 0.02). Conclusions These findings suggest considerable variability of serum hepcidin levels in HD patients. Inflammation and the use of iron did not impact on the degree of variability, and hepcidin levels were higher after an inter-dialytic period of 3 versus 2 days. These findings need to be taken into account in future studies assessing the utility of serum hepcidin as a guide to the use of iron or erythropoiesis-stimulating agents therapy. [ABSTRACT FROM PUBLISHER]
Details
- Language :
- English
- ISSN :
- 09310509
- Volume :
- 27
- Issue :
- 10
- Database :
- Complementary Index
- Journal :
- Nephrology Dialysis Transplantation
- Publication Type :
- Academic Journal
- Accession number :
- 83337764
- Full Text :
- https://doi.org/10.1093/ndt/gfs164