A New Vesicular Scaffolding Complex Mediates the G-Protein-Coupled 5-HT1A Receptor Targeting to Neuronal Dendrites.

Although essential for their neuronal function, the molecular mechanisms underlying the dendritic targeting of serotonin G-proteincoupled receptors are poorly understood. Here, we characterized a Yif1B-dependent vesicular scaffolding complex mediating the intracellular traffic of the rat 5-HT_1A receptor (5-HT_1AR) toward dendrites. By combining directed mutagenesis, GST-pull down, and surface plasmon resonance, we identified a tribasic motif in the C-tail of the 5-HT_1AR on which Yif1B binds directly with high affinity (KD37 nM). Moreover, we identified Yip1A, Rab6, and Kif5B as new partners of the 5-HT_1AR/Yif1B complex, and showed that their expression in neurons is also crucial for the dendritic targeting of the 5-HT_1AR. Live videomicroscopy revealed that 5-HT_1AR, Yif1B, Yip1A, and Rab6 traffic in vesicles exiting the soma toward the dendritic tree, and also exhibit bidirectional motions, sustaining their role in 5-HT_1AR dendritic targeting. Hence, we propose a new trafficking pathway model in which Yif1B is the scaffold protein recruiting the 5-HT_1AR in a complex including Yip1A and Rab6, with Kif5B and dynein as two opposite molecular motors coordinating the traffic of vesicles along dendritic microtubules. This targeting pathway opens new insights for G-protein-coupled receptors trafficking in neurons. [ABSTRACT FROM AUTHOR]