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An involvement of neurokinin-1 receptor in FcεRΙ-mediated RBL-2H3 mast cell activation.
- Source :
- Inflammation Research; Nov2012, Vol. 61 Issue 11, p1257-1263, 7p
- Publication Year :
- 2012
-
Abstract
- Objective and design: To determine whether the neurokinin-1 receptor (NK1R) plays a role in the activation of RBL-2H3 mast cells after FcεRΙ aggregation. Materials and methods: NK1R expression in RBL-2H3 cells was inhibited by small hairpin RNA (shRNA) against NK1R, and determined by western blotting. For activation, both NK1R knockdown and control RBL-2H3 cells were sensitized by dinitrophenol (DNP)-specific IgE and stimulated with the antigen DNP-bovine serum albumin (BSA). Following the activation of RBL-2H3 cells, monocyte chemoattractant protein (MCP-1) production and intracellular calcium flux were monitored by ELISA and confocal microscopy assay, respectively. For investigation of the signaling mechanism, phosphorylation of mitogen-activated protein kinases (MAPKs) after RBL-2H3 cell activation was assessed by western blotting. Results: shRNA-NK1R mediated an effective inhibition of NK1R expression in RBL-2H3 cells. Protein production of MCP-1 was reduced by more than 55 % in NK1R knockdown RBL-2H3 cells compared with control RBL-2H3 cells. In addition, both calcium mobilization and phosphorylation levels of MAPKs (Erk1/2, JNK, and p38) after DNP-BSA stimulation (via FcεRΙ) were decreased due to the inhibition of NK1R expression. Conclusion: NK1R is required for the activation of RBL-2H3 cells following FcεRΙ engagement and involved in the regulation of MAPK signaling pathways. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10233830
- Volume :
- 61
- Issue :
- 11
- Database :
- Complementary Index
- Journal :
- Inflammation Research
- Publication Type :
- Academic Journal
- Accession number :
- 82504060
- Full Text :
- https://doi.org/10.1007/s00011-012-0523-x