Association of the mi R-146a C>G, mi R-149T> C, mi R-196a2 T> C, and mi R-499 A> G Polymorphisms with Risk of Spontaneously Aborted Fetuses.

Problem The mi R-196a2 T> C and mi R-499 A> G polymorphisms have been reported to be genetic risk factors for recurrent spontaneous abortion; however, that previous study focused on the genetic analyses of pregnant women rather than aborted fetuses. Because annexin A1 is a target of mi R-196a2 and is related to anti-inflammation, mi R-196a2 may be immunologically important. Moreover, mi R-146a, mi R-149, mi R-196a2, and mi R-499 have shown associations with immune responses. Method of study One hundred and eighty-two spontaneously aborted fetuses ( SAFs) were <20 weeks of gestational age. The control subjects were 101 healthy children and 302 adults collected from a convenience sample. Polymerase chain reaction and restriction fragment length polymorphism analysis was performed to identify the mi R-146a C> G, mi R-149 T> C, mi R-196a2 T> C, and mi R-499 A> G genotypes. Results Chromosomally normal SAFs had significantly different frequencies of the mi R-196a2 CC, mi R-146a CC/ mi R-196a2 CC, and mi R-149 TT/ mi R-196a2 CC genotypes compared with control subjects. Conclusions mi R-196a2 CC, mi R-146a CC/ mi R-196a2 CC, and mi R-149 TT/ mi R-196a2 CC in fetuses are possible risk factors for spontaneous abortion. [ABSTRACT FROM AUTHOR]