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Development of the Fc-III Tagged Protein Expression System for Protein Purification and Detection.
- Source :
- PLoS ONE; Aug2012, Vol. 7 Issue 8, Special section p1-6, 6p
- Publication Year :
- 2012
-
Abstract
- In the present work, we developed the Fc-III tagged protein expression system for protein purification and detection. The Fc-III sequence encodes for a 13 residue peptide and this peptide is cyclized by disulfide bond formation when the fusion protein is expressed. The Fc-III-fusion proteins selectively bind to immunoglobulin Fc domains (IgG-Fc) expressed from E. coli. We showed the efficient purification of Fc-III tagged proteins by immobilized non-native IgG- Fc and the detection of the cellular locations of fusion proteins by fluorescent-conjugated IgG-Fc. Our results prove that Fc-III tagged protein expression system is a simple and efficient tool for protein purification and detection and is a useful addition to the biochemistry and proteomics toolbox. [ABSTRACT FROM AUTHOR]
- Subjects :
- PROTEINS
PEPTIDES
IMMUNOGLOBULINS
ESCHERICHIA coli
BIOCHEMISTRY
PROTEOMICS
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 7
- Issue :
- 8
- Database :
- Complementary Index
- Journal :
- PLoS ONE
- Publication Type :
- Academic Journal
- Accession number :
- 80435682
- Full Text :
- https://doi.org/10.1371/journal.pone.0044208