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Crystallization and Preliminary X-Ray Diffraction Studies of N-Acyl-D-glucosamine 2-Epimerase from Porcine Kidney.
- Source :
- Journal of Biochemistry; 1996, Vol. 120 Issue 3, p481-482, 2p
- Publication Year :
- 1996
-
Abstract
- N-Acyl-D-glucosamine 2-epimerase from porcine kidney, which was cloned and expressed in Escherichia coli, was crystallized by the vapor-diffusion method, using polyethylene glycol and ammonium acetate as precipitants. The crystals were resistant to X-ray radiation damage and diffracted to more than 2.0 Å resolution. The diffraction pattern indicated that the crystals belong to the orthorhombic system, space group P21,21 21 with unit-cell dimensions of a=78.1, b=97.2, and c=100.7 Å. It is supposed that the asymmetric unit consists of two N-acyl-D-glucosamine 2-epimerase molecules. Collection of data on the native crystals indicated that they are suitable for X-ray structural analysis. [ABSTRACT FROM AUTHOR]
- Subjects :
- GLUCOSAMINE
HEXOSAMINES
KIDNEYS
ENTEROBACTERIACEAE
GRAM-negative bacteria
Subjects
Details
- Language :
- English
- ISSN :
- 0021924X
- Volume :
- 120
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 80117300
- Full Text :
- https://doi.org/10.1093/oxfordjournals.jbchem.a021437