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Prediction and identification of mouse cytotoxic T lymphocyte epitopes in Ebola virus glycoproteins.
- Source :
- Virology Journal; 2012, Vol. 9 Issue 1, p111-117, 7p, 2 Color Photographs, 3 Charts
- Publication Year :
- 2012
-
Abstract
- Background: Ebola viruses (EBOVs) cause severe hemorrhagic fever with a high mortality rate. At present, there are no licensed vaccines or efficient therapies to combat EBOV infection. Previous studies have shown that both humoral and cellular immune responses are crucial for controlling Ebola infection. CD8+ T cells play an important role in mediating vaccine-induced protective immunity. The objective of this study was to identify H-2<superscript>d</superscript>-specific T cell epitopes in EBOV glycoproteins (GPs). Results: Computer-assisted algorithms were used to predict H-2<superscript>d</superscript>-specific T cell epitopes in two species of EBOV (Sudan and Zaire) GP. The predicted peptides were synthesized and identified in BALB/c mice immunized with replication-deficient adenovirus vectors expressing the EBOV GP. Enzyme-linked immunospot assays and intracellular cytokine staining showed that the peptides RPHTPQFLF (Sudan EBOV), GPCAGDFAF and LYDRLASTV (Zaire EBOV) could stimulate splenoctyes in immunized mice to produce large amounts of interferon-gamma. Conclusion: Three peptides within the GPs of two EBOV strains were identified as T cell epitopes. The identification of these epitopes should facilitate the evaluation of vaccines based on the Ebola virus glycoprotein in a BALB/c mouse model. [ABSTRACT FROM AUTHOR]
- Subjects :
- EBOLA virus
T cells
HEMORRHAGIC fever
CELLULAR immunity
IMMUNE response
CD8 antigen
Subjects
Details
- Language :
- English
- ISSN :
- 1743422X
- Volume :
- 9
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Virology Journal
- Publication Type :
- Academic Journal
- Accession number :
- 80034330
- Full Text :
- https://doi.org/10.1186/1743-422X-9-111