Oxidative damage and OGG1 expression induced by a combined effect of titanium dioxide nanoparticles and lead acetate in human hepatocytes.

Titanium dioxide (TiO₂) is a widely used nanomaterial that can cause biological damage through oxidative stress. At low concentrations, TiO₂ can interact with lead acetate (PbAc) to produce different toxic responses, compared with TiO₂ or PbAc alone. In this study, we utilized the following as indicators of toxic responses in human embryo hepatocytes (L02): reactive oxygen species (ROS), reduced glutathione (GSH), superoxide dismutase (SOD), and the DNA adducts 8-hydroxydeoxyguanosine (8-OHdG) and 8-oxoguanine DNA glycosylase homolog 1 (OGG1). These were used to evaluate the oxidative stress of TiO₂ (at 0.001, 0.01, 0.1, 1, and 10 μg mL⁻¹) mixed with PbAc (1 μg mL⁻¹) on L02 cells without photoactivation. Compared with the negative control (1‰ dimethyl sulfoxide), TiO₂ mixed with PbAc induced increased release of ROS (at 0.001, 0.01, 0.1, 1, 10 μg mL⁻¹ TiO₂), intracellular SOD activity (at 0.1 and 0.01 μg mL⁻¹ TiO₂), GSH levels (at 0.01-1 μg mL⁻¹ TiO₂), 8-OHdG levels (at 1 and 10 μg mL⁻¹ TiO₂), OGG1 expression (at 0.001-1 μg mL⁻¹ TiO₂), and cytotoxicity (at 0.1, 1, and 10 μg mL⁻¹ TiO₂) in L02 cells. There were no significant changes in ROS, GSH, SOD, 8-OHdG, or OGG1 levels when L02 cells were treated with TiO₂ alone or PbAc alone. These findings indicate that TiO₂ and PbAc in combination induce cytotoxicity and oxidative stress in L02 cells in the absence of photoactivation. © 2011 Wiley Periodicals, Inc. Environ Toxicol, 2012. [ABSTRACT FROM AUTHOR]