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Placental Mitochondrial DNA Content and Particulate Air Pollution during in Utero Life.
- Source :
- Environmental Health Perspectives; Sep2012, Vol. 120 Issue 9, p1346-1352, 7p, 4 Charts, 1 Graph
- Publication Year :
- 2012
-
Abstract
- Background: Studies emphasize the importance of particulate matter (PM) in the formation of reactive oxygen species and inflammation. We hypothesized that these processes can influence mitochondrial function of the placenta and fetus. Objective: We investigated the influence of PM<subscript>10</subscript> exposure during pregnancy on the mitochondrial DNA content (mtDNA content) of the placenta and umbilical cord blood. Methods: DNA was extracted from placental tissue (n = 174) and umbilical cord leukocytes (n = 176). Relative mtDNA copy numbers (i.e., mtDNA content) were determined by real-time polymerase chain reaction. Multiple regression models were used to link mtDNA content and in utero exposure to PM<subscript>10</subscript> over various time windows during pregnancy. Results: In multivariate-adjusted analysis, a 10-μg/m³ increase in PM10 exposure during the last month of pregnancy was associated with a 16.1% decrease [95% confidence interval (CI): -25.2, -6.0%, p = 0.003] in placental mtDNA content. The corresponding effect size for average PM<subscript>10</subscript> exposure during the third trimester was 17.4% (95% CI: -31.8, -0.1%, p = 0.05). Furthermore, we found that each doubling in residential distance to major roads was associated with an increase in placental mtDNA content of 4.0% (95% CI: 0.4, 7.8%, p = 0.03). No association was found between cord blood mtDNA content and PM<subscript>10</subscript> exposure. Conclusions: Prenatal PM<subscript>10</subscript> exposure was associated with placental mitochondrial alterations, which may both reflect and intensify oxidative stress production. The potential health consequences of decreased placental mtDNA content in early life must be further elucidated. [ABSTRACT FROM AUTHOR]
- Subjects :
- DNA analysis
REACTIVE oxygen species
AIR pollution
APGAR score
CONFIDENCE intervals
STATISTICAL correlation
CORD blood
INFLAMMATION
MITOCHONDRIA
PLACENTA
QUESTIONNAIRES
RESEARCH funding
STATISTICS
DATA analysis
MULTIPLE regression analysis
OXIDATIVE stress
PARTICULATE matter
DATA analysis software
DESCRIPTIVE statistics
FETUS
Subjects
Details
- Language :
- English
- ISSN :
- 00916765
- Volume :
- 120
- Issue :
- 9
- Database :
- Complementary Index
- Journal :
- Environmental Health Perspectives
- Publication Type :
- Academic Journal
- Accession number :
- 79839029
- Full Text :
- https://doi.org/10.1289/ehp.1104458