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Effect of Streptococcus sanguinis/ Porphyromonas gingivalis single and combined biofilms upon platelet aggregation.
- Source :
- Oral Diseases; Sep2012, Vol. 18 Issue 6, p586-594, 9p, 1 Color Photograph, 2 Charts, 5 Graphs
- Publication Year :
- 2012
-
Abstract
- Oral Diseases (2012) 18, 586-594 Objective: To assess the effect of two oral bacteria Streptococcus sanguinis and Porphyromonas gingivalis upon platelet aggregation. Materials and Methods: Streptococcus sanguinis, P. gingivalis, S. sanguniis + P. gingivalis were added to platelet-rich plasma and platelet aggregation measured using a platelet aggregometer. Platelets were passed through a flow chamber with S. sanguinis, P. gingivalis or a biofilm of S. sanguinis and P. gingivalis coated with saliva. Platelet adhesion to the chamber was observed under a fluorescence microscope for 15 min. The positive control was platelets treated with adrenaline; the negative control was platelets treated with phosphate-buffered saline. Results: The mean (± s.e.) aggregation magnitude of S. sanguinis and P. gingivalis was 77.7 ± 7.4% and 79.3 ± 9.9%, respectively. The aggregation magnitude of S. sanguinis + P. gingivalis was 51.3 ± 12.9%, which was significantly lower than that for S. sanguinis/ P. gingivalis ( P < 0.05). In the flow chamber system, platelets adhered to S. sanguinis/P.gingivalis respectively within 3 min, and reached a plateau at 5-15 min. Under the condition of the S. sanguinis- and P. gingivalis-saliva biofilm, platelet adhesion to the biofilm was significantly reduced at 5-15 min ( P < 0.05). Conclusions: In the static or dynamic flow system, platelets adhered to S. sanguinis or P. gingivalis. However, if S. sanguinis was mixed with P. gingivalis, the aggregation magnitude (%) was significantly reduced. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 1354523X
- Volume :
- 18
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Oral Diseases
- Publication Type :
- Academic Journal
- Accession number :
- 78333991
- Full Text :
- https://doi.org/10.1111/j.1601-0825.2012.01913.x