Expressing PHB synthetic genes through chloroplast genetic engineering.

Discusses research with chloroplast integration and expression vector in tobacco. Construction of expression cassettes for phbB, phbA, phbC and aadA genes, which were bombarded into the tobacco u chloroplast genome; Analysis of tTransplastomic plants with PCR and Southern blot; Examination of their homoplastomy; Investigation of transgene expression and transcriptional level with northern dot and RT-PCR analysis; Compatibility of the chloroplast transformation system for poly-3-hydroxybutyrate (PHB) production.