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Studies on HOXB4 Expression During Differentiation of Human Cytomegalovirus-infected Hematopoietic Stem Cells into Lymphocyte and Erythrocyte Progenitor Cells.
- Source :
- Cell Biochemistry & Biophysics; Jun2012, Vol. 63 Issue 2, p133-141, 9p
- Publication Year :
- 2012
-
Abstract
- We investigated the role of homeobox B4 (HOXB4) mRNA/protein expression induced by human cytomegalovirus (HCMV) and/or all-trans retinoic acid (ATRA) in proliferation and committed differentiation of human cord blood hematopoietic stem cells (HSCs) into colony-forming-units of T-lymphocyte (CFU-TL) and erythroid (CFU-E) progenitors in vitro. Twelve cord blood samples were collected from the fetal placenta umbilical vein and cultured in vitro. The proliferation and differentiation of cord blood HSCs into CFU-TL and CFU-E were continuously disrupted with HCMV-AD169 and/or 6 × 10 mol/l of ATRA. HOXB4 mRNA/protein expression in CFU-TL and CFU-E was detected in control, ATRA, HCMV and ATRA + HCMV groups on days 3, 7, and 12 of culture by fluorescent qRT-PCR/western blot. We found that HOXB4 mRNA/protein expression was detectable on day 3, increased on day 7 and was highest on day 12. HOXB4 mRNA/protein expression in HCMV group was downregulated compared with control group ( P < 0.05). However, the levels were significantly upregulated in HCMV + ATRA group compared with HCMV group ( P < 0.05). We concluded that the abnormal HOXB4 mRNA/protein expression induced by HCMV could play a role in hematopoietic damage. ATRA, at the concentration used, significantly up-regulated HOXB4 mRNA/protein expression in normal lymphocyte and erythrocyte progenitor cells as well as in HCMV-infected cells. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10859195
- Volume :
- 63
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Cell Biochemistry & Biophysics
- Publication Type :
- Academic Journal
- Accession number :
- 75231685
- Full Text :
- https://doi.org/10.1007/s12013-012-9349-y