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Preparation of human scFv antibody against nasopharyngeal carcinoma and identification of its specificity.
- Source :
- Acta Oto-Laryngologica; Jun2012, Vol. 132 Issue 6, p663-670, 8p, 3 Color Photographs, 2 Black and White Photographs, 1 Diagram, 1 Chart
- Publication Year :
- 2012
-
Abstract
- Conclusion: The selected scFv antibody could specifically recognize and target nasopharyngeal carcinoma (NPC), and could be applied to clinical diagnosis and therapy. Objective: The aim was to construct and screen fully human anti-NPC single chain Fv fusion phage libraries, and to identify the specificity of the scFv antibody. Methods: Peripheral blood mononuclear cells of patients with NPC were immunized in vitro by NPC cells and transformed by Epstein-Barr virus. The total RNAwas used to construct the scFv libraries. By means of ELISA and immunochemistry, the positively bound scFv was selected and identified. The positive scFv was fused to EGFP, and was then expressed in E. coli strain BL21 (DE3) and purified. Furthermore, we observed the binding bioactivity. Results: The fusion protein has the biological activity of binding the NPC cells and emitting green fluorescence. In targeting experiments in vivo, the results showed that the fusion protein can successfully target the NPC. [ABSTRACT FROM AUTHOR]
- Subjects :
- CANCER diagnosis
CANCER treatment
THERAPEUTIC use of monoclonal antibodies
NASOPHARYNX tumors
DNA analysis
BIOLOGICAL assay
BIOPHYSICS
CELL culture
ELECTROPHORESIS
ENZYME-linked immunosorbent assay
IMMUNOHISTOCHEMISTRY
RESEARCH methodology
MICE
MONOCLONAL antibodies
POLYMERASE chain reaction
RECOMBINANT proteins
RESEARCH funding
TISSUE culture
DATA analysis software
DIAGNOSIS
TUMOR treatment
Subjects
Details
- Language :
- English
- ISSN :
- 00016489
- Volume :
- 132
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Acta Oto-Laryngologica
- Publication Type :
- Academic Journal
- Accession number :
- 75179734
- Full Text :
- https://doi.org/10.3109/00016489.2012.655861