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Hoechst 33342-induced autophagy protected HeLa cells from caspase-independent cell death with the participation of ROS.
- Source :
- Free Radical Research; Jun2012, Vol. 46 Issue 6, p740-749, 10p
- Publication Year :
- 2012
-
Abstract
- Autophagy, an evolutionarily-conserved intracellular organelle and protein degradation process, may exhibit drastically different effects on cell survival depending on the particular environmental and culturing conditions. Hoechst 33342 (HO), a fluorescent dye widely used for staining DNA, has been reported to induce apoptosis in mammalian cells. Here we showed that, in addition to caspase-independent cell death, HO also induced autophagy in HeLa cells, as evidenced by the accumulation of autophagosomes, LC3 form conversion and LC3 puncta formation in a cell line stably expressing GFP-LC3. HO treatment led to generation of reactive oxygen species (ROS), and inhibition of ROS with N-acetyl- l-cysteine (NAC) abrogated both autophagy and caspase-independent cell death. Finally, autophagy played a protective role against caspase-independent cell death, as cell death induced by HO was enhanced under pharmacological and siRNA-mediated genetic inhibition of autophagy. [ABSTRACT FROM AUTHOR]
- Subjects :
- AUTOPHAGY
HELA cells
CASPASES
REACTIVE oxygen species
SPECIES
CELL death
HOECHST 33258
Subjects
Details
- Language :
- English
- ISSN :
- 10715762
- Volume :
- 46
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Free Radical Research
- Publication Type :
- Academic Journal
- Accession number :
- 75062792
- Full Text :
- https://doi.org/10.3109/10715762.2012.670701