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Effect of pretreatment of hydrothermally processed rice straw with laccase-displaying yeast on ethanol fermentation.
- Source :
- Applied Microbiology & Biotechnology; May2012, Vol. 94 Issue 4, p939-948, 10p
- Publication Year :
- 2012
-
Abstract
- A gene encoding laccase I was identified and cloned from the white-rot fungus Trametes sp. Ha1. Laccase I contained 10 introns and an original secretion signal sequence. After laccase I without introns was prepared by overlapping polymerase chain reaction, it was inserted into expression vector pULD1 for yeast cell surface display. The oxidation activity of a laccase-I-displaying yeast as a whole-cell biocatalyst was examined with 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), and the constructed yeast showed a high oxidation activity. After the pretreatment of hydrothermally processed rice straw (HPRS) with laccase-I-displaying yeast with ABTS, fermentation was conducted with yeast codisplaying endoglucanase, cellobiohydrolase, and β-glucosidase with HPRS. Fermentation of HPRS treated with laccase-I-displaying yeast was performed with 1.21-fold higher activities than those of HPRS treated with control yeast. The results indicated that pretreatment with laccase-I-displaying yeast with ABTS was effective for direct fermentation of cellulosic materials by yeast codisplaying endoglucanase, cellobiohydrolase, and β-glucosidase. [ABSTRACT FROM AUTHOR]
- Subjects :
- POLYMERASE chain reaction
LACCASE
YEAST
ETHANOL
FERMENTATION
Subjects
Details
- Language :
- English
- ISSN :
- 01757598
- Volume :
- 94
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Applied Microbiology & Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 74638020
- Full Text :
- https://doi.org/10.1007/s00253-012-3876-8