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Characterization of a thermostable β-glucosidase from Aspergillus fumigatus Z5, and its functional expression in Pichia pastoris X33.

Source :
Microbial Cell Factories; 2012, Vol. 11 Issue 1, p25-39, 15p, 4 Diagrams, 5 Charts, 2 Graphs
Publication Year :
2012

Abstract

The article offers information on a study that characterized thermostable native beta-glucosidase (nBgl3) from Aspergillus fumigatus Z5 and shown its expression in Pichia pastoris X33. For this nBgl3 was purified to electrophoretic homogeneity and their sequence were obtained. Biochemical properties of purified nBgl3 and recombinant Bgl3 were determined and their gene was expressed in Pichia pastoris.

Details

Language :
English
ISSN :
14752859
Volume :
11
Issue :
1
Database :
Complementary Index
Journal :
Microbial Cell Factories
Publication Type :
Academic Journal
Accession number :
74101664
Full Text :
https://doi.org/10.1186/1475-2859-11-25