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Vaccination of Lactating Dairy Cows for the Prevention of Aflatoxin B1 Carry Over in Milk.
- Source :
- PLoS ONE; 2011, Vol. 6 Issue 10, p1-9, 9p
- Publication Year :
- 2011
-
Abstract
- The potential of anaflatoxin B<subscript>1</subscript> (AnAFB<subscript>1</subscript>) conjugated to keyhole limpet hemocyanin (KLH) as a vaccine (AnAFB<subscript>1</subscript>-KLH) in controlling the carry over of the aflatoxin B<subscript>1</subscript> (AFB<subscript>1</subscript>) metabolite aflatoxin M1 (AFM<subscript>1</subscript>) in cow milk is reported. AFB<subscript>1</subscript> is the most carcinogenic compound in food and foodstuffs amongst aflatoxins (AFs). AnAFB<subscript>1</subscript> is AFB<subscript>1</subscript> chemically modified as AFB<subscript>1</subscript>-1(O-carboxymethyl) oxime. In comparison to AFB<subscript>1</subscript>, AnAFB<subscript>1</subscript> has proven to be non-toxic in vitro to human hepatocarcinoma cells and non mutagenic to Salmonella typhimurium strains. AnAFB<subscript>1</subscript>-KLH was used for immunization of cows proving to induce a long lasting titer of anti-AFB<subscript>1</subscript> IgG antibodies (Abs) which were cross reactive with AFB<subscript>1</subscript>, AFG<subscript>1</subscript>, and AFG<subscript>2</subscript>. The elicited anti-AFB<subscript>1</subscript> Abs were able to hinder the secretion of AFM<subscript>1</subscript> into the milk of cows continuously fed with AFB<subscript>1</subscript>. Vaccination of lactating animals with conjugated AnAFB<subscript>1</subscript> may represent a solution to the public hazard constituted by milk and cheese contaminated with AFs. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 6
- Issue :
- 10
- Database :
- Complementary Index
- Journal :
- PLoS ONE
- Publication Type :
- Academic Journal
- Accession number :
- 73890941
- Full Text :
- https://doi.org/10.1371/journal.pone.0026777