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Deleterious effects of fungizone on growth hormone and prolactin secretion by cultured GH cells.
- Source :
- In Vitro Cellular & Developmental Biology; Dec1987, Vol. 23 Issue 12, p837-840, 4p
- Publication Year :
- 1987
-
Abstract
- The rates at which growth hormone (GH) and prolactin (PRL) are spontaneously secreted from a rat pituitary tumor cell line (GH) were significantly reduced when these cells were maintained in medium containing 2.5 μg/ml Fungizone (Fz). The reduction in hormone secretion was not immediately reversed by removal of Fz during perifusion, but after 3 wk in control medium, secretory rates approached the pre-Fz treatment levels. In plated cells, secretion of GH was reduced by Fz in a dose-dependent manner, whereas PRL secretion was significantly reduced only by the highest concentration (2.5 μg/ml) of Fz. We concluded that Fz is not an acceptable medium constituent for the long-term culture of GH cells. However, because its effects are reversible, its short-term use as a decontaminating agent might eliminate the necessity for reinitiating the culture of cells whose secretory behavior must be followed in long-term protocols. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 08838364
- Volume :
- 23
- Issue :
- 12
- Database :
- Complementary Index
- Journal :
- In Vitro Cellular & Developmental Biology
- Publication Type :
- Academic Journal
- Accession number :
- 73494404
- Full Text :
- https://doi.org/10.1007/BF02620962