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Isolation and long-term serial cultivation of endothelial cells from the microvessels of the adult human dermis.
- Source :
- In Vitro; Dec1983, Vol. 19 Issue 12, p937-945, 9p
- Publication Year :
- 1983
-
Abstract
- A method to isolate and maintain microvascular endothelial cells from the cutaneous vessels of adult human skin in long-term culture has been developed. Endothelial cells lining the microvessels of the papillary dermis are released from surrounding tissue during a brief trypsin incubation (0.3% trypsin, 1% EDTA). Cells are plated onto a fibronectin substrate and maintained in Leibovitz (L15) culture medium containing pooled human serum (50%) and antibiotics. Proliferation is dependent upon the presence of several additional growth factors, cholera enterotoxin (1×10 M), isobutyl methylxanthine (3.3×10 M), and medium conditioned by explant culture of the mouse EHS sarcoma. Using this supplemented medium, cells proliferate readily and can be cultivated serially for more than 6 passages (3 months in vitro). These cells retain their characteristic endothelial cell morphology, stain positively for Factor VIII antigen, and contain Weibel-Palade bodies. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00735655
- Volume :
- 19
- Issue :
- 12
- Database :
- Complementary Index
- Journal :
- In Vitro
- Publication Type :
- Academic Journal
- Accession number :
- 73493886
- Full Text :
- https://doi.org/10.1007/BF02661715