Oxygen limitation on L-serine production in a hollow-fiber bioreactor.

Pseudomonas AM1 utilizes glycine and methanol to produce l-serine aerobically (1). The consumption of methanol in this bioconversion is stoichiometrically in excess of L-serine production (2). Consequently, the oxygen requirement associated with L-serine production is higher than expected for the conversion from glycine. One method of L-serine production investigated was a technique utilizing a hollow-fiber ultrafiltration cartridge as a bioreactor. Oxygen diffusion limitations appear to impede the consumption of methanol and, consequently, the production of L-serine in such a reactor. Methanol consumption data agree with predictions based on a hollow-fiber diffusion model. [ABSTRACT FROM AUTHOR]