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Genetic analysis of Salmonella enteritidis biofilm formation: critical role of cellulose.

Authors :
Solano, Cristina
García, Begoña
Valle, Jaione
Berasain, Carmen
Ghigo, Jean-Marc
Gamazo, Carlos
Lasa, Iñigo
Source :
Molecular Microbiology; Feb2002, Vol. 43 Issue 3, p793-808, 16p
Publication Year :
2002

Abstract

Summary We report here a new screening method based on the fluorescence of colonies on calcofluor agar plates to identify transposon insertion mutants of Salmonella enteritidis that are defective in biofilm development. The results not only confirmed the requirement of genes already described for the modulation of multicellular behaviour in Salmonella typhimurium and other species, but also revealed new aspects of the biofilm formation process, such as two new genetic elements, named as bcs ABZC and bcs EFG operons, required for the synthesis of an exopolysaccharide, digestible with cellulase. Non-polar mutations of bcs C and bcs E genes and complementation experiments demonstrated that both operons are respon-sible for cellulose biosynthesis in both S. enteritidis and S. typhimurium . Using two different growth media, ATM and LB, we showed that the biofilm produced by S. enteritidis is made of different constituents, suggesting that biofilm composition and regulation depends on environmental conditions. Bacterial adherence and invasion assays of eukaryotic cells and in vivo virulence studies of cellulose-deficient mutants indicated that, at least under our experimental conditions, the production of cellulose is not involved in the virulence of S. enteritidis . However, cellulose-deficient mutants were more sensitive to chlorine treatments, suggesting that cellulose production and biofilm formation may be an important factor for the survival of S. enteritidis on surface environments. [ABSTRACT FROM AUTHOR]

Subjects

Subjects :
SALMONELLA enteritidis
BIOFILMS

Details

Language :
English
ISSN :
0950382X
Volume :
43
Issue :
3
Database :
Complementary Index
Journal :
Molecular Microbiology
Publication Type :
Academic Journal
Accession number :
7297341
Full Text :
https://doi.org/10.1046/j.1365-2958.2002.02802.x