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Influence of inorganic phosphate and organic buffers on cephalosporin production by Streptomyces clavuligerus.
- Source :
- Archives of Microbiology; Nov1977, Vol. 115 Issue 2, p169-173, 5p
- Publication Year :
- 1977
-
Abstract
- A high concentration of potassium phosphate (75-100 mM) stabilized pH and supported extensive growth of Streptomyces clavuligerus in a chemically defined medium; such a concentration also inhibited cephalosporin production. Although Tris buffer was found to have detrimental effects on growth and antibiotic production, 3-(N-morpholino)-propane sulfonate (MOPS) or 2-(N-morpholino)-ethane sulfonate (MES) buffer provided a nontoxic buffering system. In the presence of MOPS buffer, cephalosporin production was optimal at 25 mM phosphate, whereas higher concentrations of phosphate progressively inhibited antibiotic production up to 85% without modifying the pH pattern. MOPS buffer can be used to conduct fermentations at a relatively constant pH value in shake flasks. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 03028933
- Volume :
- 115
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Archives of Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 72939227
- Full Text :
- https://doi.org/10.1007/BF00406371