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Quinidine inhibition of the muscarine receptor-activated K channel current in atrial cells of guinea pig.
- Source :
- Naunyn-Schmiedeberg's Archives of Pharmacology; 1987, Vol. 335 Issue 2, p216-218, 3p
- Publication Year :
- 1987
-
Abstract
- Postsynaptic mechanisms underlying the anticholinergic effects of quinidine were examined in single atrial cells, using the tight-seal whole-cell recording technique. The solution in the glass pipettes contained guanosine-5′triphosphate (GTP) or guanosine-5′-O-(3-thiotriphosphate) (GTP-γS, a non-hydrolyzable GTP analogue). In both cases, acetylcholine (ACh), applied to the bath, induced a specific K current. In GTP-loaded cells, quinidine in the bath solution depressed the ACh-induced K current concentration-dependently. Atropine also blocked the K current. On the other hand, in GTP-γS-loaded cells, the ACh-induced current was not blocked by atropine and persisted even when ACh was washed out from the bath, indicating that GTP-γS causes uncoupling of the K channels from the muscarine receptors. Quinidine, however, did depress the increased K current concentration-dependently. The percent inhibition curves for quinidine to depress the K current were very similar between GTP-loaded and GTP-γS-loaded cells. From these observations, we suggest that direct inhibition of the muscarine receptor-activated K channel current by quinidine, and not blockade of the muscarine receptor itself, is mainly responsible for the anticholinergic effects of the drug in atrial myocytes. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00281298
- Volume :
- 335
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Naunyn-Schmiedeberg's Archives of Pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 72632656
- Full Text :
- https://doi.org/10.1007/BF00177726