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Protective Effects of Aucubin on H2O2-Induced Apoptosis in PC12 Cells.
- Source :
- Phytotherapy Research; Mar2012, Vol. 26 Issue 3, p369-374, 6p
- Publication Year :
- 2012
-
Abstract
- The present study investigated the neuroprotective effects of aucubin on hydrogen peroxide (H<subscript>2</subscript>O<subscript>2</subscript>)-induced apoptosis in PC12 cells. Exposure of PC12 cells to 0.25 m m H<subscript>2</subscript>O<subscript>2</subscript> induced a leakage of lactate dehydrogenase and decreased cell viability, as shown by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. In a dose over 0.1 m m, aucubin increased PC12 cellular viability and markedly attenuated H<subscript>2</subscript>O<subscript>2</subscript>-induced apoptotic cell death. Quantitation of apoptosis by flow cytometry indicated that aucubin inhibited H<subscript>2</subscript>O<subscript>2</subscript>-induced apoptosis in PC12 cells. Nuclear damage was alleviated by aucubin, as shown by Hoechst staining. In addition, the levels of malondialdehyde were reduced and the activity of superoxide dismutase, catalase and glutathione peroxidase was augmented in these cells. These results indicated that aucubin inhibited H<subscript>2</subscript>O<subscript>2</subscript>-induced apoptosis in PC12 cells through regulation of the endogenous oxidant-antioxidant balance. Our results suggest that aucubin is a potential protective agent for the treatment of oxidative-stress-induced neurodegenerative disease. Copyright © 2011 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 0951418X
- Volume :
- 26
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Phytotherapy Research
- Publication Type :
- Academic Journal
- Accession number :
- 72458263
- Full Text :
- https://doi.org/10.1002/ptr.3562