A technique for recording the activity of single interneurons from free-moving crickets ( Gryllus campestris L.).

Techniques for the production of a suction electrode assembly, and its attachment to a cricket ( Gryllus campestris) in such a way that the cricket can walk freely while the activity of individual interneurons in the cervical connective is clearly observable, are described. The suction electrode (tip diameter of 100 to 200 μm) is placed in contact with the surface of a connective (or peripheral nerve) and suction is applied. If the response of the unit of interest is clearly observable above background activity, the electrode assembly is attached to a supporting metal collar, which has been previously attached to the cricket. With the electrode thus supported, the cricket is released, walks freely, courts, copulates and in general behaves normally. Some examples of unit-recordings obtained from interneurons in the cervical connective of free-moving crickets are shown. [ABSTRACT FROM AUTHOR]