Back to Search Start Over

Detection of collagenase mRNA in odontoclasts of bovine root-resorbing tissue by in situ hybridization.

Authors :
Okamura, Takeshi
Shimokawa, Hitoyata
Takagi, Yuzo
Ono, Hiroshi
Sasaki, Satoshi
Okamura, T
Shimokawa, H
Takagi, Y
Ono, H
Sasaki, S
Source :
Calcified Tissue International; 1993, Vol. 52 Issue 4, p325-330, 6p
Publication Year :
1993

Abstract

The odontoclast, which is morphologically similar to osteoclast, is thought to play a major role in root resorption of deciduous teeth. High collagenolytic activity has been detected in the root resorbing tissue. In order to identify collagenase-producing cells and the role of collagenase in root resorption of deciduous tooth, in situ hybridization of collagenase mRNA in bovine root-resorbing tissue sections was performed using a digoxigenin-labeled, nonradioactive RNA probe. Collagenase mRNA expression was clearly observed in odontoclasts in addition to the macrophages, fibroblasts, odontoblasts, and cementoblasts. Multinuclear odontoclasts showed intense tartrate-resistant acid phosphatase (TRAP) activity. We also examined interleukin-1 (IL-1) mRNA expression in the root-resorbing tissue by in situ hybridization. IL-1 transcripts were found to be expressed in odontoclasts, fibroblasts, and macrophages suggesting that IL-1 might be an important factor for promoting root resorption. These results suggest that the collagenase produced by odontoclasts may play a key role in dentin collagen degradation in root resorption. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
0171967X
Volume :
52
Issue :
4
Database :
Complementary Index
Journal :
Calcified Tissue International
Publication Type :
Academic Journal
Accession number :
72389025
Full Text :
https://doi.org/10.1007/BF00296659