Studies on the amino-acid metabolism of Mycobacterium tuberculosis H37Rv.

(i) Data are presented on the amino-acid composition of (i) the clear culture filtrate, (ii) the culture filtrate hydrolysate, (iii) the clear cell homogenate, (iv) the cell homogenate hydrolysate, and (v) the insoluble protein mass of Mycobacterium tuberculosis H37Rv at different periods of growth. Data also are presented on the amino-acid composition of the three tuberculin proteins isolated from the culture filtrate of a fully grown culture of Myco. tuber. H37Rv. (ii) From among the fractions studied, it is concluded that the cell homogenate and its hydrolysate are most indicative of the amino-acid changes taking place within the cell. [ABSTRACT FROM AUTHOR]