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Nucleotide sequence of cDNA clones encoding the β subunit of mitochondrial ATP synthase from the green alga Chlamydomonas reinhardtii: The precursor protein encoded by the cDNA contains both an N-terminal presequence and a C-terminal extension.

Authors :
Franzén, Lars-Gunnar
Falk, Gunnar
Source :
Plant Molecular Biology; Aug1992, Vol. 19 Issue 5, p771-780, 10p
Publication Year :
1992

Abstract

cDNA and genomic clones encoding the β subunit of mitochondrial ATP synthase from Chlamydomonas reinhardtii have been isolated using heterologous DNA probes from the photosynthetic bacterium Rhodospirillum rubrum. The protein encoded by the cDNA is 79-83% identical to corresponding proteins from higher-plant and mammalian mitochondria, and 75% identical to the R. rubrum protein. It contains both an N-terminal presequence and a unique C-terminal extension. The presequence, which is the first mitochondrial presequence determined in C. reinhardtii, is similar in structure to mitochondrial presequences from other organisms. As chloroplast presequences from C. reinhardtii also share features with mitochondrial presequences from other organisms (L.-G. Franzén et al., FEBS Lett 260 (1990) 165-168), this raises interesting questions about protein targeting to chloroplasts and mitochondria in C. reinhardtii. The possibility that the C-terminal extension is involved in targeting the protein to the mitochondrion is discussed. Southern blot analysis indicates that the protein is encoded by a single-copy gene. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
01674412
Volume :
19
Issue :
5
Database :
Complementary Index
Journal :
Plant Molecular Biology
Publication Type :
Academic Journal
Accession number :
71756047
Full Text :
https://doi.org/10.1007/BF00027073