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Molecular diversity of three S-allele cDNAs associated with gametophytic self-incompatibility in Lycopersicon peruvianum.
- Source :
- Plant Molecular Biology; Oct1994, Vol. 26 Issue 2, p757-762, 6p
- Publication Year :
- 1994
-
Abstract
- We isolated S allele-associated cDNA clones from each of the stylar cDNA libraries of Lycopersicon peruvianum of two different S genotypes ( SSand S S) with S Sallele-associated cDNA (LPS11) as a probe. The longest cDNA clones, designated LPS12 and LPS13, which were 779 bp and 853 bp in length, contained open reading frames of 189 and 210 amino acids, respectively. The three S alleleassociated cDNAs (LPS11, LPS12, and LPS13) did not cross-hybridize to each other under highly stringent condition by northern blot analysis. Their average identity to Nicotiana alata S-proteins so far was 49%. The fragments corresponding to LPS11 or LPS12 cosegregated with their respective S alleles in genetic crosses. From these results, we conclude that the three cloned cDNAs were derived from the three different S alleles of L. peruvianum. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01674412
- Volume :
- 26
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Plant Molecular Biology
- Publication Type :
- Academic Journal
- Accession number :
- 71461131
- Full Text :
- https://doi.org/10.1007/BF00013760