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Molecular diversity of three S-allele cDNAs associated with gametophytic self-incompatibility in Lycopersicon peruvianum.

Authors :
Chung, Il-Kyung
Ito, Toru
Tanaka, Hiroshi
Ohta, Akinori
Nan, Hong
Takagi, Masamichi
Source :
Plant Molecular Biology; Oct1994, Vol. 26 Issue 2, p757-762, 6p
Publication Year :
1994

Abstract

We isolated S allele-associated cDNA clones from each of the stylar cDNA libraries of Lycopersicon peruvianum of two different S genotypes ( SSand S S) with S Sallele-associated cDNA (LPS11) as a probe. The longest cDNA clones, designated LPS12 and LPS13, which were 779 bp and 853 bp in length, contained open reading frames of 189 and 210 amino acids, respectively. The three S alleleassociated cDNAs (LPS11, LPS12, and LPS13) did not cross-hybridize to each other under highly stringent condition by northern blot analysis. Their average identity to Nicotiana alata S-proteins so far was 49%. The fragments corresponding to LPS11 or LPS12 cosegregated with their respective S alleles in genetic crosses. From these results, we conclude that the three cloned cDNAs were derived from the three different S alleles of L. peruvianum. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
01674412
Volume :
26
Issue :
2
Database :
Complementary Index
Journal :
Plant Molecular Biology
Publication Type :
Academic Journal
Accession number :
71461131
Full Text :
https://doi.org/10.1007/BF00013760