The sequences of two nuclear genes and a pseudogene for tRNA from the higher plant Phaseolus vulgaris.

A genomic bank of nuclear DNA (nDNA) from the higher plant Phaseolus vulgaris, constructed using the lambda EMBL-4 vector, has been screened for the presence of tRNA genes. One of the many positive recombinants was found to hybridise several times stronger than the other positives, and has been shown to contain several tRNA genes. We report the structure of two nuclear tRNA genes for tRNA, namely tRNA(UGG) and tRNA(AGG), and that of a 'pseudogene' for tRNA. This 'pseudogene', despite showing 95% homology with the other tRNA species presented here, has several features which are likely to affect its transcription or its functioning as a tRNA. [ABSTRACT FROM AUTHOR]