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Wild chromosomal variants in Aspergillus nidulans.

Authors :
Geiser, David
Arnold, Michael
Timberlake, William
Source :
Current Genetics; Feb1996, Vol. 29 Issue 3, p293-300, 8p
Publication Year :
1996

Abstract

Pulsed-field gel electrophoresis and a chromosome-specific cosmid DNA library were used to determine the karyotypes of wild-type Aspergillus nidulans isolates from around the world. Overall, little structural variation was found, with a few major exceptions. One isolate possessed a non-essential B-chromosome of about 1.0 million base pairs (mb). Another isolate had undergone a non-reciprocal translocation of about 1.6 mb of chromosome VI onto chromosome VIII. Other than these chromosomal differences, these isolates appeared phenotypically normal. To analyze its effects on meiosis, the translocation isolate was outcrossed with another wild-type derivative that had a 'normal' electrophoretic karyotype. This cross produced a range of phenotypes, including duplicated progeny that had a 'barren' phenotype similar to that described for Neurospora partial disomics. The duplication was somewhat vegetatively unstable. This is the first association of sterility with chromosomal duplication in A. nidulans. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
01728083
Volume :
29
Issue :
3
Database :
Complementary Index
Journal :
Current Genetics
Publication Type :
Academic Journal
Accession number :
71296082
Full Text :
https://doi.org/10.1007/BF02221561