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The SNQ3 gene of Saccharomyces cerevisiae confers hyper-resistance to several functionally unrelated chemicals.
- Source :
- Current Genetics; Jun1991, Vol. 19 Issue 6, p429-433, 5p
- Publication Year :
- 1991
-
Abstract
- A multi-copy plasmid containing the SNQ3 gene confers hyper-resistance to 4-nitroquinoline-N-oxide (4NQO), Trenimon, MNNG, cycloheximide, and to sulfometuron methyl in yeast transformants. Restriction analysis, subcloning, and DNA sequencing revealed an open reading frame of 1950 bp on the SNQ3-containing insert DNA. Gene disruption and transplacement into chromosomal DNA yielded 4NQO-sensitive null mutants which were also more sensitive than the wild-type to Trenimon, cycloheximide, sulfometuron methyl, and MNNG. Hydropathic analysis showed that the SNQ3-encoded protein is most likely not membrane-bound, while the codon bias index points to low expression of the gene. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 01728083
- Volume :
- 19
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Current Genetics
- Publication Type :
- Academic Journal
- Accession number :
- 71293718
- Full Text :
- https://doi.org/10.1007/BF00312733