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Case report: T-cell responses during clearance of andes virus from blood cells 2 months after severe hantavirus cardiopulmonary syndrome.

Authors :
Manigold, Tobias
Martinez, Jessica
Lazcano, Ximena
Ye, Chunyan
Schwartz, Shaina
Cuiza, Analía
Valdivieso, Francisca
Hjelle, Brian
Vial, Pablo
Source :
Journal of Medical Virology; Nov2008, Vol. 80 Issue 11, p1947-1951, 5p
Publication Year :
2008

Abstract

Hantavirus Cardiopulmonary Syndrome (HCPS) due to Andes virus (ANDV) is endemic in Chile and Argentina and currently demonstrates a case-fatality rate of 37% in humans. By contrast to the chronically infected rodents, it is believed that ANDV in humans is cleared during the acute phase. Moreover, to date, both magnitude and quality of human T-cell responses during ANDV infection and clearance are unknown. Using IFN-γ and granzyme B ELISPOT assays as well as flow cytometry, we prospectively studied the ANDV-specific T-cell responses in a 56-year-old convalescing survivor of severe HCPS, whose blood cells remained PCR-positive for ANDV-RNA until day 53 after hospital admission, that is, 67 days after infection and 42 days after discharge. PCR-negativity was closely related to the increase and function of (Gn<subscript>46-60</subscript>)-specific IFN-γ<superscript>+</superscript> granzyme B<superscript>+</superscript> CD8<superscript>+</superscript> T-cells, but not to neutralizing antibody titers. Concurrently, the phenotype of CD45RA<superscript>+</superscript>CCR7<superscript>−</superscript> Gn<subscript>46-60</subscript>-specific T-cells shifted from a CD28<superscript>−</superscript>CD27<superscript>+</superscript> 'intermediate' to a CD28<superscript>−</superscript> CD27<superscript>−</superscript> 'late' effector memory beyond day 53 after hospital admission. This is the first report that shows that ANDV can persist in the human hosts for more than 2 months. Moreover, the kinetics of T-cell responses during ANDV clearance may indicate a major role of T-cells for clearance of ANDV and human immunity to this pathogen. J. Med. Virol. 80:1947-1951, 2008. © 2008 Wiley-Liss, Inc. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
01466615
Volume :
80
Issue :
11
Database :
Complementary Index
Journal :
Journal of Medical Virology
Publication Type :
Academic Journal
Accession number :
71245081
Full Text :
https://doi.org/10.1002/jmv.21302