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Transgenic poplars: expression of chimeric genes using four different constructs.
- Source :
- Plant Cell Reports; Apr1992, Vol. 11 Issue 3, p137-141, 5p
- Publication Year :
- 1992
-
Abstract
- Leaf or stem explants of a hybrid poplar clone ( Populus tremula X Populus alba), sensitive to Agrobacterium tumefaciens, were co-cultivated either by an octopine or a nopaline disarmed A. tumefaciens modified strain. Transformed poplar shoots were readily regenerated from explants. The protocol was improved using the nopaline disarmed strain C58/pMP90 with the binary vector pBI121. This protocol was then used to test three other vectors. The first one, possessing a nptII gene fused to the CaMV 19S promoter, permitted regeneration of transformed shoots in presence of 50 to 100 mg/l kanamycin. The two other vectors carried an additional nptII gene under the control of the CaMV 35S or CaMV 35S promoter with a double enhancer sequence (CaMV 70). CaMV 70 promoter provided consistently higher level of gene expression than the other promoters in both callus and leaf tissues. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 07217714
- Volume :
- 11
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- Plant Cell Reports
- Publication Type :
- Academic Journal
- Accession number :
- 70886066
- Full Text :
- https://doi.org/10.1007/BF00232166