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Diagnosis of human immunodeficiency virus infection by immunoassay using a molecularly cloned and expressed virus envelope polypeptide. Comparison to Western blot on 2707 consecutive serum samples.

Authors :
Burke, Donald S.
Brandt, Brenda L.
Redfield, Robert R.
Lee, Tun-Hou
Thorn, Richard M.
Beltz, Gerald A.
Hung, Chung-Ho
Burke, D S
Brandt, B L
Redfield, R R
Lee, T H
Thorn, R M
Beltz, G A
Hung, C H
Source :
Annals of Internal Medicine; May87, Vol. 106 Issue 5, p671-676, 6p
Publication Year :
1987

Abstract

To detect human immunodeficiency virus (HIV) antibodies in a simple enzyme-linked immunoassay (CBre3-EIA), we used an Escherichia coli-expressed polypeptide antigen, representing the carboxy-terminal third of the external membrane glycoprotein gene fused with the amino-terminal half of the transmembrane glycoprotein gene. Over a 3-month period, 2707 consecutive serum samples referred for confirmatory testing for human T-lymphotrophic virus type III (HTLV-III) antibodies were evaluated by both Western blot and CBre3-EIA. On a single determination for each sample, the CBre3-EIA was found to have an estimated sensitivity (99.9%) and specificity (99.1%) similar or superior to the more cumbersome Western blot method. This study shows that all HIV-seropositive subjects have antibodies to the virus envelope protein; no other virus antigens are required for construction of highly sensitive immunoassays. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00034819
Volume :
106
Issue :
5
Database :
Complementary Index
Journal :
Annals of Internal Medicine
Publication Type :
Academic Journal
Accession number :
7001726
Full Text :
https://doi.org/10.7326/0003-4819-106-5-671