A proteomic approach for the identification of novel lysine methyltransferase substrates.

The article presents information on a study on over 9,500 human proteins and developed and optimized a system for proteome-wide identification of novel methylation events that was catalyzed by the protein lysine methyltransferase (PKMT) SETD6. The study identified novel candidate substrates for SETD6 by using two independent detection approaches The study also verified that all targets tested in vitro as well as in cells were genuine substrates. The study described a novel proteome-wide methodology for identifying the new PKMT substrates.