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An atypical component of RNA-directed DNA methylation machinery has both DNA methylation-dependent and -independent roles in locus-specific transcriptional gene silencing.
- Source :
- Cell Research; Dec2011, Vol. 21 Issue 12, p1691-1700, 10p
- Publication Year :
- 2011
-
Abstract
- RNA-directed DNA methylation (RdDM) is an important de novo DNA methylation pathway in plants. RdDM mediates the transcriptional silencing of many endogenous genomic loci, most of which are transposon related. A forward genetics screen identified DTF1 (DNA-binding transcription factor 1) as a new component for RdDM in Arabidopsis. Loss-of-function mutations in DTF1 release the transcriptional silencing of RdDM target loci and reduce the accumulation of 24-nt small interfering RNAs (siRNAs) from some of the targets. Interestingly, in the dtf1 mutant plants, the release of transcriptional gene silencing at solo-LTR is accompanied by decreased siRNA accumulation but not by reduced DNA methylation. These results suggest that DTF1 is an atypical component of RdDM and has both DNA methylation-dependent and -independent roles in transcriptional gene silencing. We suggest that besides DNA methylation, siRNAs may cause some other uncharacterized epigenetic modifications that lead to transcriptional gene silencing. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10010602
- Volume :
- 21
- Issue :
- 12
- Database :
- Complementary Index
- Journal :
- Cell Research
- Publication Type :
- Academic Journal
- Accession number :
- 67526776
- Full Text :
- https://doi.org/10.1038/cr.2011.173