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Hypovolemic state: age-related influence of water restriction on cardiac nitric oxide synthase in rats.
- Source :
- European Journal of Nutrition; Dec2011, Vol. 50 Issue 8, p657-664, 8p, 1 Diagram, 2 Charts, 4 Graphs
- Publication Year :
- 2011
-
Abstract
- Aim of study: We have assessed the influence of water restriction stress on the nitric oxide (NO) synthase in heart and aorta tissues in young 2-month-old and middle-aged 12-month-old rats. Methods: Animals were divided into control and 24- and 72-h water-deprived groups. We evaluated systolic blood pressure (SBP), biochemical parameters, nitrate and nitrite urinary excretion (UNOx), NADPH-diaphorase activity, and protein levels of NOS in the right atria, left ventricle, and thoracic aorta tissues. Results: Water restriction during 72 h increased SBP (16%) in 2-month-old rats but decreased it after 24 and 72 h (9 and 15%, respectively) in 12-month-old rats. Atria, aorta endothelium, and smooth muscle NOS activity increased (32, 63, and 88%, respectively) only after 72 h of water restriction in 2-month-old rats. It also increased not only after 72 h but also after 24 h in atria (27 and 18%, respectively) and in ventricle (39 and 67%, respectively) in 12-month-old rats. Meanwhile, in this group's aorta smooth muscle, the enzyme activity decreased (16 and 7%, respectively). A major difference seen between ages was the changes in UNOx excretion, which decreased in the younger in 24 and 72 h (47 and 81%, respectively) and increased in the middle-aged rats (193 and 389%, respectively). Water restriction did not change cardiovascular endothelial and neuronal NOS protein levels in any group. Conclusion: NO pathways could contribute to the development of age-related cardiovascular adaptation to volume depletion induced by water restriction. [ABSTRACT FROM AUTHOR]
- Subjects :
- PROTEIN metabolism
AGE distribution
ANALYSIS of variance
ANIMAL experimentation
AORTA
BIOPHYSICS
BLOOD testing
BLOOD pressure
CARDIOVASCULAR system physiology
COMPARATIVE studies
DEHYDRATION
LEFT heart ventricle
HEART atrium
HEMODYNAMICS
HISTOLOGICAL techniques
RESEARCH methodology
MICROSCOPY
NITRATES
NITRIC oxide
NITRITES
RATS
RESEARCH funding
STATISTICAL sampling
SMOOTH muscle
STATISTICS
WESTERN immunoblotting
DATA analysis
REPEATED measures design
DATA analysis software
Subjects
Details
- Language :
- English
- ISSN :
- 14366207
- Volume :
- 50
- Issue :
- 8
- Database :
- Complementary Index
- Journal :
- European Journal of Nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 67186949
- Full Text :
- https://doi.org/10.1007/s00394-011-0183-6